For the Susa, Carnoy, or Formol sublimate, what is the dehydration sequence for absolute alcohol?

The dehydration sequence for absolute alcohol in histopathology is centered around the use of higher concentrations of alcohol to effectively dehydrate tissue samples before embedding them in paraffin or using other embedding media. In this process, absolute alcohol is typically used due to its effectiveness in removing water from the tissues.

The correct approach is to immerse the tissue in absolute alcohol for 1-2 hours, repeating this process three times. This ensures thorough dehydration, which is essential for optimal tissue processing. The dehydration must be done meticulously because insufficient dehydration can lead to issues in tissue infiltration and embedding, which would ultimately affect the quality of the histological sections.

Using absolute alcohol multiple times allows for a consistent and effective removal of moisture from the tissue, thereby facilitating better infiltration of embedding mediums in subsequent steps.

While other choices mention the use of varying concentrations or durations, they do not provide the optimal and specific protocol for using absolute alcohol, which seeks to achieve maximum dehydration efficiency in the given time frame.