How long does the paraffin wax application take in Grauper's method?

In Grauper's method, the paraffin wax application typically takes about 45 minutes. This duration is optimal for ensuring that the tissue samples are adequately infiltrated with wax, which is essential for providing structural support during the subsequent slicing of the tissue into thin sections. The paraffin embedding process is crucial in histopathology, as it preserves the morphology of the tissue, allowing for proper analysis under the microscope. Inadequate time in paraffin infiltration could result in poor tissue preservation and compromised histological evaluation. Therefore, the 45-minute duration strikes a balance between efficiency and thoroughness in the embedding process.