What is the dehydration sequence for Bouin's fluid?

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The dehydration sequence for Bouin's fluid involves several key steps, with the primary goal of properly dehydrating the tissue specimens before embedding them in paraffin. Option B, which states "3-12 hours in 70%-90% alcohol," is correct because the dehydration phase typically involves immersing the samples in progressively higher concentrations of alcohol to remove water from the tissue while preserving cellular structure and morphology.

When tissues are placed in Bouin's fluid, they are initially fixed and then require proper dehydration to prevent distortion during the embedding process. The 70%-90% alcohol provides a gentler means of dehydration, allowing for more gradual removal of water compared to using absolute alcohol right away. This method minimizes the risk of tissue damage and ensures better preservation of the histological features.

Following this dehydration step, the next phase usually involves treatment with absolute alcohol before transitioning to other solutions necessary for embedding, such as xylene. The specific period of 3-12 hours is also practical because it allows adequate dehydration without over-processing, which could lead to the destruction of delicate tissue structures crucial for histopathological examination.

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