Which of the following acids is specifically used for tissue decalcification?

When considering the decalcification of tissue samples in histopathology, it is important to note that various acids can be employed for this process, including nitric acid, trichloroacetic acid, and formic acid. Each of these acids works effectively to remove calcium deposits from tissues, thereby facilitating subsequent histological examination.

Aqueous nitric acid is particularly valuable due to its ability to rapidly decalcify tissue while preserving cellular morphology. It achieves this via a reaction with calcium salts, converting them into soluble forms that can be easily washed away.

Trichloroacetic acid is another option and is often chosen for its moderate decalcifying capabilities. It’s effective for tissues that may be sensitive to stronger acids, allowing for a balance between decalcification and preservation of tissue integrity.

Formic acid solution is also widely used in this context, praised for its gentler action compared to stronger acids. It offers the advantage of being less likely to cause tissue distortion while still effectively removing calcium, making it a popular choice in many histopathological laboratories.

Given the effectiveness of all three acids in decalcifying tissues, stating that all of them are specifically used for tissue decalcification encompasses the range of options available for pathologists when preparing